Using stem cell suspension culture methods and biology, human adult spinal disc stem cells were isolated and monoclonally cultured into multicellular sphere-like clusters discospheres. Discospheres from the irst culture series were collected, processed, and replated as single stem cells for serial expansion studies using suspension culture, demonstrating linear expansion was possible. Discospheres and adult spinal disc stem cells were plated on matrix coated culture surfaces in stem cell media for several hours to allow ixation, and assayed for the stem cell biomarkers. Discospheres and adult spinal disc stem cells were plated on laminin-coated culture surfaces in chondrogenic media and culture conditions for 14 days to differentiate them into NP cells.
PY - Y1 - N2 - The availability of quantitative techniques for assessment of stem cell function is central to the development of methods for stem cell isolation. Any approach to identification and isolation of adult stem cells can be no better than the assay used to detect function.
The degree of expansion that occurs from stem cell to progenitor cell to mature cell is vast enough to prevent absolute distinction between the most primitive stem cells and the differentiating progeny of these cells. Methods for hematopoietic stem cell HSC isolation described in the chapter include approaches that minimize coisolation of nonstem cells, while also providing techniques to isolate populations of progenitor cells possessing remarkable proliferative potential in the absence of stem cell activity.
Comparison of primitive hematopoietic stem cells with early progenitor cells will provide interesting insights into the early stages of hematopoietic development.
Although transplanted progenitor cells may provide sufficient hematopoietic function to rescue animals from hematopoietic failure after radiation conditioning, it is necessary to utilize markers to distinguish blood cells derived from the donor graft from those of recipient origin in order to definitively demonstrate HSC function.
The combination of negative with positive magnetic selection is a powerful technique for rapid enrichment of a mixture of hematopoietic stem and progenitor cells. These cells may be used directly in studies evaluating hematopoietic function in vivo or in vitro, or may be further processed to achieve higher purity of functionally distinct subsets of HSCs or progenitor cells.Isolation, characterization, and in vitro proliferation of canine mesenchymal stem cells derived from bone marrow, adipose tissue, muscle, and periosteum.
The main purpose of this study was to isolate and characterize gingival connective tissue-derived mesenchymal stem cells (GMSCs).
The secondary purpose . -of-the-art regarding isolation, characterization and aging of adipose tissue-derived mesenchymal stem cells (ADSCs).
Mesenchymal stem cells (MSCs) have recently received. Sep 02, · Isolation and characterization of endosteal niche cell populations that regulate hematopoietic stem cells.
|Book subject areas||PY - Y1 - N2 - Obesity is a complex, multifactorial disease that has been extensively researched in recent times.|
|Cell isolation - Wikipedia||Additionally, isolated populations of germline cells having different phenotypes are disclosed wherein the subpopulations are capable of forming long-term cultures of multipotent or pluripotent cells or are capable of differentiating into mature germline cells and repopulating a sterile reproductive organ.|
|Neural Stem Cell Isolation and Characterization - ScienceDirect||Jun 20, Abstract Infectious diseases remain a major threat to public health.|
|Isolation and Characterization of Hematopoietic Stem Cells — University of Utah||Numerous studies have been conducted to understand the mechanisms that regulate ASC proliferation, growth and differentiation, however standard protocols about harvesting and processing techniques are not yet defined.|
|Characterization of Human Mesenchymal Stem Cells Isolated from the Testis||Lee; Anandi Sawant, Ph.|
Yuka Nakamura, Fumio Arai, Hiroko Iwasaki, Kentaro Hosokawa, Isolation of cell fractions adhering to the endosteum. By contrast, the uterus can be accessed nonsurgically, and may provide a more readily available cell source. While human endometrium is known to harbor mesenchymal precursor cells, MSCs have not been identified in equine endometrium.
This study reports the isolation, culture, and characterization of MSCs from equine endometrium. Abstract. Hematopoietic cells differentiate in steps marked by the acquisition or loss of specific phenotypic characteristics. Human bone marrow cells that were responsive to the early-acting cytokines Kit ligand and interleukin-3 were forced to a metabolic death.